Maintenance project | cycle |
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Check the tubing and fittings to see if the tubing is wrinkled | every week, or if the flow rate is not accurate, the fitting has leaks and there are air bubbles in the tubing |
Replace piping and fittings | annually |
Check the xenon lamp energy | every quarter, or when the detector noise is high and the sensitivity is reduced |
Replace the xenon lamp | When the xenon lamp is not lit or the xenon lamp energy is not enough. |
Cleaning the flow cell | quarterly |
Confirm that the solvent bottle is clean | daily |
Problems | Possible Causes | Solutions |
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Baseline noise | Detection pool window contamination |
1、Flush the detection cell with 1 mol/L nitric acid, water and new solvent 2、Remove the detection pool, open the cleaning or replace the pool window quartz |
Bubbles in the sample cell | 1、Suddenly increase the flow to drive out the bubbles. 2、Add a BPR at the exit end of the test cell to increase the pressure inside the test cell. |
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Detector light source failure | 1、Check the xenon lamp setting status 2、Check the lamp usage time, lamp energy, and number of starts 3、Replace the xenon lamp |
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Solvent leakage | Tighten or replace the connector | |
Small bubbles pass through the detection cell | 1、The mobile phase is carefully degassed 2、Increase the back pressure of the test cell 3、System leak detection |
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Particles passing through the detection pool | 1、Cleaning the detection pool 2、Check the separation column outlet sieve |
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Baseline drift | Detection pool window contamination | 1、Flush the detection cell with 1 mol/L nitric acid, water and new solvent 2、Remove the detection pool, open the cleaning or replace the pool window quartz |
Detector temperature change | System constant temperature | |
Detector light source failure | Replace xenon lamp | |
The original mobile phase is not completely removed. | Rinse the system with a new mobile phase to replace the solvent, or replace it with a compatible solvent. | |
Solvent storage bottle contamination | Clean solvent bottles with new mobile phase balancing system | |
Strongly adsorbed components are eluted from the separation column | 1. Wash the separation column with a solvent with strong elution ability before the next separation. 2. Using solvent gradient |
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Large spikes appear in the curve | Bubbles pass through the test cell. | 1. Degas the solvent and thoroughly flush the system. 2. Check if the connection system leaks |
Negative peak | Injection failure | Use the injection valve to verify that there are no air bubbles in the sample loop during injection |
The mobile phase used is impure. | Use a chromatographically pure mobile phase or purify the solvent. | |
The curve panel signal rises stepwise; the flat head peak; the baseline cannot be returned to zero. | The output range of the detector is not set properly. | Reset the output range of the detector. |
As the pump reciprocates, there is a spike in the detection tank. | Bubbles in the detection cell | Remove the inlet tube of the detection tank from the joint of the separation column, and use a syringe to push methanol from the outlet end to remove air bubbles. |
Problems | Possible Causes | Solutions |
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The pump is running, but no solvent is output. | The solvent reservoir is empty. | Fill the reservoir. |
The actual flow rate is lower than the set value. | The filter head is dirty. | Clean or replace the filter head. |
The gradient valve has dirt. | Clean or replace the gradient valve. | |
The pipe has wrinkles. | Replace the pipe. | |
No solvent is delivered. | Pump is not running, power switch is not turned on. | Turn on the Power switch. |
The solvent is placed lower than the pump head | Move the solvent to a position higher than the pump head or horizontal position with the pump head | |
The pressure rises too high. | The pipe is blocked. | Find the blockage and handle it. |
The inner diameter of the pipe is too small. | Replace the appropriate inner diameter line | |
Online filter blocking | Stainless steel screens for cleaning or replacing inline filters | |
Column blocking | Replace column | |
Flow cell blockage | Cleaning flow cell | |
Pump stops during operation | The pressure exceeds the high pressure limit. | 1、Reset the maximum pressure limit. 2、Replace the column 3、Replace the appropriate inner diameter pipeline 4、 Cleaning the flow cell |
Pump overheat protection | View fan and circuit | |
The pump flow rate becomes smaller | The air bubbles in the pump gather. | Open the pump head and the pipeline, let the pump run at high flow rate, and eliminate the air bubbles. |
The solvent filter is clogged. | Open the pump head inlet pressure cap. If the solvent does not flow out of the infusion tube very quickly, the filter is clogged and needs to be cleaned or replaced. | |
The two solutions in the pump are not mutually soluble. | A solvent is used between the two solvents to dissolve the two mutually insoluble solvents. | |
Plunger seal leak | Replace the plunger seal | |
Strongly adsorbed components are eluted from the separation column | Bubbles accumulate in the pump head. | Let the pump run at high flow rates, eliminate bubbles. |
Solvent stratification in the pump | Using a transition solvent to dissolve the two | |
The pump is not tightly sealed. | Tighten the fixing screws. | |
The infusion line is leaking or partially blocked | Check the pipeline one by one to eliminate | |
The pump has a click and cannot start normally | Motor failure | Stop the pump and check |
Line voltage is too low | Increase line voltage | |
Excessive pressure | See "Pressure is too high" | |
The column pressure is too high | The stigma is blocked by impurities | Disassemble the column head and clean the column head filter. If the impurity particles have entered the bed , carefully remove the sediment and the contaminated packing, and then fill in the same packing, do not leave a gap in the column head; The method is to add a filter in front of the column. |
There is column pressure after pumping, but no mobile phase flows out of the detector. | Serious leakage in the system. | Repair the injection valve or the tubing and fasteners between the pump and the detector |
Flow path blockage | Clear the inlet of the injector, the injection line or the connecting line between the column and the detector or the particles of the detector | |
The inlet end of the column is blocked by particles | Clean or replace the column inlet filter; replace one column as needed; filter all samples and solvent | |
Increased column pressure and reduced flow | The inlet of the detector or detector is partially blocked | Disassemble and clean the test cell and tubing |